human t84 colon epithelial cells Search Results


human epithelial colonic carcinoma cell line t84  (DS Pharma Biomedical)
90
DS Pharma Biomedical human epithelial colonic carcinoma cell line t84
Human Epithelial Colonic Carcinoma Cell Line T84, supplied by DS Pharma Biomedical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human epithelial colonic carcinoma cell line t84/product/DS Pharma Biomedical
Average 90 stars, based on 1 article reviews
human epithelial colonic carcinoma cell line t84 - by Bioz Stars, 2026-03
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human t 84 colonic epithelial cells  (Mediatech)
90
Mediatech human t 84 colonic epithelial cells
Spermidine increases TCPTP expression at the protein level in T84 and HT29 intestinal <t>epithelial</t> cells. A, SPD (0.1, 1.0, and 10 μm) increased TCPTP protein levels in T84 (A) and HT29 cells (B) in a dose-dependent manner following 24 h treatment (n = 4). Unt, untreated. C, SPD (0.1, 1.0, 10 μm) showed no effect on PTPN2 mRNA expression in T84 cells following 24-h treatment (n = 6). PTPN2 mRNA expression was normalized to the housekeeping gene GAPDH. D, cycloheximide (CHX, 35.5 μm) significantly reduced TCPTP protein levels following 24 h treatment. Coadministration of SPD (10 μm) reversed this effect (n = 4). Whole cell lysates from SPD and CHX-treated cells were obtained, and TCPTP protein levels were assessed by Western blot analysis. β-Actin was used throughout as a loading control, and the relative protein levels were assessed by densitometry. All data are expressed as a percentage of the control ± S.E. *, p < 0.05 compared with untreated control; ###, p < 0.001 compared with CHX-treated cells; Student's unpaired t test or ANOVA and Student-Newman-Keuls post hoc test.
Human T 84 Colonic Epithelial Cells, supplied by Mediatech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human t 84 colonic epithelial cells/product/Mediatech
Average 90 stars, based on 1 article reviews
human t 84 colonic epithelial cells - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


Spermidine increases TCPTP expression at the protein level in T84 and HT29 intestinal epithelial cells. A, SPD (0.1, 1.0, and 10 μm) increased TCPTP protein levels in T84 (A) and HT29 cells (B) in a dose-dependent manner following 24 h treatment (n = 4). Unt, untreated. C, SPD (0.1, 1.0, 10 μm) showed no effect on PTPN2 mRNA expression in T84 cells following 24-h treatment (n = 6). PTPN2 mRNA expression was normalized to the housekeeping gene GAPDH. D, cycloheximide (CHX, 35.5 μm) significantly reduced TCPTP protein levels following 24 h treatment. Coadministration of SPD (10 μm) reversed this effect (n = 4). Whole cell lysates from SPD and CHX-treated cells were obtained, and TCPTP protein levels were assessed by Western blot analysis. β-Actin was used throughout as a loading control, and the relative protein levels were assessed by densitometry. All data are expressed as a percentage of the control ± S.E. *, p < 0.05 compared with untreated control; ###, p < 0.001 compared with CHX-treated cells; Student's unpaired t test or ANOVA and Student-Newman-Keuls post hoc test.

Journal: The Journal of Biological Chemistry

Article Title: Spermidine Stimulates T Cell Protein-tyrosine Phosphatase-mediated Protection of Intestinal Epithelial Barrier Function *

doi: 10.1074/jbc.M113.475962

Figure Lengend Snippet: Spermidine increases TCPTP expression at the protein level in T84 and HT29 intestinal epithelial cells. A, SPD (0.1, 1.0, and 10 μm) increased TCPTP protein levels in T84 (A) and HT29 cells (B) in a dose-dependent manner following 24 h treatment (n = 4). Unt, untreated. C, SPD (0.1, 1.0, 10 μm) showed no effect on PTPN2 mRNA expression in T84 cells following 24-h treatment (n = 6). PTPN2 mRNA expression was normalized to the housekeeping gene GAPDH. D, cycloheximide (CHX, 35.5 μm) significantly reduced TCPTP protein levels following 24 h treatment. Coadministration of SPD (10 μm) reversed this effect (n = 4). Whole cell lysates from SPD and CHX-treated cells were obtained, and TCPTP protein levels were assessed by Western blot analysis. β-Actin was used throughout as a loading control, and the relative protein levels were assessed by densitometry. All data are expressed as a percentage of the control ± S.E. *, p < 0.05 compared with untreated control; ###, p < 0.001 compared with CHX-treated cells; Student's unpaired t test or ANOVA and Student-Newman-Keuls post hoc test.

Article Snippet: Tissue Culture Human T 84 colonic epithelial cells (passages 20–40) were grown in 1:1 Dulbecco's modified Eagle's medium/Ham's F-12 medium with l -glutamine and 15 m m HEPES (Mediatech Inc., Manassas, VA), supplemented with 5% newborn calf serum (Invitrogen) and 1% penicillin/streptomycin (Cellgro/Mediatech, Herndon, VA) at 37 °C in 5% CO 2 .

Techniques: Expressing, Western Blot, Control

Spermidine increases TCPTP enzymatic activity in T84 and HT29 intestinal epithelial cells. T84 (A) and HT29 (B) cells were treated with SPD (10 μm) for 30 min. TCPTP was immunoprecipitated from whole cell lysates, and TCPTP activity was assessed (n = 3). A sample from each immunoprecipitation was probed for TCPTP by Western blotting to confirm equal protein loading. Fluorescence activity units were compared with TCPTP protein levels to account for any differences in overall phosphatase amounts. All data are expressed as a percentage of the control ± S.E. *, p < 0.05; **, p < 0.01 compared with the respective untreated time point; Student's unpaired t test.

Journal: The Journal of Biological Chemistry

Article Title: Spermidine Stimulates T Cell Protein-tyrosine Phosphatase-mediated Protection of Intestinal Epithelial Barrier Function *

doi: 10.1074/jbc.M113.475962

Figure Lengend Snippet: Spermidine increases TCPTP enzymatic activity in T84 and HT29 intestinal epithelial cells. T84 (A) and HT29 (B) cells were treated with SPD (10 μm) for 30 min. TCPTP was immunoprecipitated from whole cell lysates, and TCPTP activity was assessed (n = 3). A sample from each immunoprecipitation was probed for TCPTP by Western blotting to confirm equal protein loading. Fluorescence activity units were compared with TCPTP protein levels to account for any differences in overall phosphatase amounts. All data are expressed as a percentage of the control ± S.E. *, p < 0.05; **, p < 0.01 compared with the respective untreated time point; Student's unpaired t test.

Article Snippet: Tissue Culture Human T 84 colonic epithelial cells (passages 20–40) were grown in 1:1 Dulbecco's modified Eagle's medium/Ham's F-12 medium with l -glutamine and 15 m m HEPES (Mediatech Inc., Manassas, VA), supplemented with 5% newborn calf serum (Invitrogen) and 1% penicillin/streptomycin (Cellgro/Mediatech, Herndon, VA) at 37 °C in 5% CO 2 .

Techniques: Activity Assay, Immunoprecipitation, Western Blot, Fluorescence, Control

Spermidine attenuates STAT1 and 3 phosphorylation in IFN-γ-treated T84 and HT29 intestinal epithelial cells. IFN-γ (1000 units/ml) induced phosphorylation of STAT1 and 3 in T84 (A and B) and HT29 (C and D) cells following 30-min treatment. Coadministration of SPD (10 μm) for this time significantly attenuated IFN-γ-induced phosphorylation of STAT1 and 3 in both T84 and HT29 cells (n = 3–5). Whole cell lysates from IFN-γ and/or SPD-treated T84 and HT29 cells were obtained, and STAT1 and 3 protein levels were assessed by Western blot analysis. β-Actin was used throughout as a loading control, and the relative protein levels were assessed by densitometry. All data are expressed as a percentage of the control ± S.E. **, p < 0.01; ***, p < 0.001 compared with untreated control; #, p < 0.05; ##, p < 0.01; ###, p < 0.001 compared with IFN-γ-treated cells; ANOVA and Student-Newman-Keuls post hoc test. Unt, untreated.

Journal: The Journal of Biological Chemistry

Article Title: Spermidine Stimulates T Cell Protein-tyrosine Phosphatase-mediated Protection of Intestinal Epithelial Barrier Function *

doi: 10.1074/jbc.M113.475962

Figure Lengend Snippet: Spermidine attenuates STAT1 and 3 phosphorylation in IFN-γ-treated T84 and HT29 intestinal epithelial cells. IFN-γ (1000 units/ml) induced phosphorylation of STAT1 and 3 in T84 (A and B) and HT29 (C and D) cells following 30-min treatment. Coadministration of SPD (10 μm) for this time significantly attenuated IFN-γ-induced phosphorylation of STAT1 and 3 in both T84 and HT29 cells (n = 3–5). Whole cell lysates from IFN-γ and/or SPD-treated T84 and HT29 cells were obtained, and STAT1 and 3 protein levels were assessed by Western blot analysis. β-Actin was used throughout as a loading control, and the relative protein levels were assessed by densitometry. All data are expressed as a percentage of the control ± S.E. **, p < 0.01; ***, p < 0.001 compared with untreated control; #, p < 0.05; ##, p < 0.01; ###, p < 0.001 compared with IFN-γ-treated cells; ANOVA and Student-Newman-Keuls post hoc test. Unt, untreated.

Article Snippet: Tissue Culture Human T 84 colonic epithelial cells (passages 20–40) were grown in 1:1 Dulbecco's modified Eagle's medium/Ham's F-12 medium with l -glutamine and 15 m m HEPES (Mediatech Inc., Manassas, VA), supplemented with 5% newborn calf serum (Invitrogen) and 1% penicillin/streptomycin (Cellgro/Mediatech, Herndon, VA) at 37 °C in 5% CO 2 .

Techniques: Phospho-proteomics, Western Blot, Control

Spermidine inhibits PI3K activation by IFN-γ and protects intestinal epithelial barrier function from inflammatory cytokine treatment. A, T84 cells (1 × 105) were seeded onto coverslips and allowed to grow for 2 days. On day 3, fresh medium (DMEM) was added, and cells were pretreated ± SPD (10 μm) for 15 min. After 15 min, the cells were treated with IFN-γ (1000 units/ml) for 15 min. PI3K activation was detected with a rabbit anti-phospho-PI3K antibody (phospho-p85Tyr-458/p55Tyr-199; 1:50 dilution; green). DNA was stained with DAPI (blue). IFN-γ treatment increased PI3K phosphorylation, and this was reduced in the presence of SPD (representative image from five fields of view, ×20, n = 2, scale bar = 200 μm). B, IFN-γ (1000 units/ml) was added basolaterally to T84 monolayers that had stable TER values of ≥ 1000 Ω/cm2. Following 24-h treatment, TER values decreased by 35% of their initial base-line measurements. Coadministration of SPD (10 μm) for this time significantly reduced the effects of IFN-γ on barrier resistance (n = 4). C, following 24-h treatment, IFN-γ (1000 units/ml) also induced increases in epithelial permeability as measured by FITC-dextran flux. Coadministration of SPD (10 μm) for this time significantly reduced the effects of IFN-γ on barrier permeability (n = 4). Data are expressed as a percentage of the untreated control ± S.E. *, p < 0.05; ***, p < 0.001 compared with untreated cells; #, p < 0.05 compared with IFN-γ-treated cells; ANOVA and Student-Newman-Keuls post hoc test. UNT, untreated.

Journal: The Journal of Biological Chemistry

Article Title: Spermidine Stimulates T Cell Protein-tyrosine Phosphatase-mediated Protection of Intestinal Epithelial Barrier Function *

doi: 10.1074/jbc.M113.475962

Figure Lengend Snippet: Spermidine inhibits PI3K activation by IFN-γ and protects intestinal epithelial barrier function from inflammatory cytokine treatment. A, T84 cells (1 × 105) were seeded onto coverslips and allowed to grow for 2 days. On day 3, fresh medium (DMEM) was added, and cells were pretreated ± SPD (10 μm) for 15 min. After 15 min, the cells were treated with IFN-γ (1000 units/ml) for 15 min. PI3K activation was detected with a rabbit anti-phospho-PI3K antibody (phospho-p85Tyr-458/p55Tyr-199; 1:50 dilution; green). DNA was stained with DAPI (blue). IFN-γ treatment increased PI3K phosphorylation, and this was reduced in the presence of SPD (representative image from five fields of view, ×20, n = 2, scale bar = 200 μm). B, IFN-γ (1000 units/ml) was added basolaterally to T84 monolayers that had stable TER values of ≥ 1000 Ω/cm2. Following 24-h treatment, TER values decreased by 35% of their initial base-line measurements. Coadministration of SPD (10 μm) for this time significantly reduced the effects of IFN-γ on barrier resistance (n = 4). C, following 24-h treatment, IFN-γ (1000 units/ml) also induced increases in epithelial permeability as measured by FITC-dextran flux. Coadministration of SPD (10 μm) for this time significantly reduced the effects of IFN-γ on barrier permeability (n = 4). Data are expressed as a percentage of the untreated control ± S.E. *, p < 0.05; ***, p < 0.001 compared with untreated cells; #, p < 0.05 compared with IFN-γ-treated cells; ANOVA and Student-Newman-Keuls post hoc test. UNT, untreated.

Article Snippet: Tissue Culture Human T 84 colonic epithelial cells (passages 20–40) were grown in 1:1 Dulbecco's modified Eagle's medium/Ham's F-12 medium with l -glutamine and 15 m m HEPES (Mediatech Inc., Manassas, VA), supplemented with 5% newborn calf serum (Invitrogen) and 1% penicillin/streptomycin (Cellgro/Mediatech, Herndon, VA) at 37 °C in 5% CO 2 .

Techniques: Activation Assay, Staining, Phospho-proteomics, Permeability, Control

Spermidine protects intestinal epithelial barrier function in a TCPTP-dependent manner. T84 cells were transfected with either TCPTP-specific or nonspecific control siRNA and allowed to grow for 48 h, at which point IFN-γ (1000 units/ml) was added basolaterally to the monolayers. Following 24 h treatment, TER values decreased on average by 8% of their initial base-line measurements. Coadministration of SPD (10 μm) for this time significantly reduced the effects of IFN-γ on barrier resistance (n = 5). The protective effect of SPD on IFN-γ-induced decreases in TER was not realized in TCPTP knockdown cells (n = 5). Whole cell lysates from IFN-γ- and/or SPD-treated T84 cells were obtained, and TCPTP protein levels were assessed by Western blot analysis. **, p < 0.01; ***, p < 0.001 compared with untreated cells; #, p < 0.05 compared with IFN-γ treatment of control siRNA-transfected cells; $$, p < 0.01 compared with IFN-γ/SPD treatment of control siRNA-transfected cells. Unt, untreated.

Journal: The Journal of Biological Chemistry

Article Title: Spermidine Stimulates T Cell Protein-tyrosine Phosphatase-mediated Protection of Intestinal Epithelial Barrier Function *

doi: 10.1074/jbc.M113.475962

Figure Lengend Snippet: Spermidine protects intestinal epithelial barrier function in a TCPTP-dependent manner. T84 cells were transfected with either TCPTP-specific or nonspecific control siRNA and allowed to grow for 48 h, at which point IFN-γ (1000 units/ml) was added basolaterally to the monolayers. Following 24 h treatment, TER values decreased on average by 8% of their initial base-line measurements. Coadministration of SPD (10 μm) for this time significantly reduced the effects of IFN-γ on barrier resistance (n = 5). The protective effect of SPD on IFN-γ-induced decreases in TER was not realized in TCPTP knockdown cells (n = 5). Whole cell lysates from IFN-γ- and/or SPD-treated T84 cells were obtained, and TCPTP protein levels were assessed by Western blot analysis. **, p < 0.01; ***, p < 0.001 compared with untreated cells; #, p < 0.05 compared with IFN-γ treatment of control siRNA-transfected cells; $$, p < 0.01 compared with IFN-γ/SPD treatment of control siRNA-transfected cells. Unt, untreated.

Article Snippet: Tissue Culture Human T 84 colonic epithelial cells (passages 20–40) were grown in 1:1 Dulbecco's modified Eagle's medium/Ham's F-12 medium with l -glutamine and 15 m m HEPES (Mediatech Inc., Manassas, VA), supplemented with 5% newborn calf serum (Invitrogen) and 1% penicillin/streptomycin (Cellgro/Mediatech, Herndon, VA) at 37 °C in 5% CO 2 .

Techniques: Transfection, Control, Knockdown, Western Blot